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Cambridge Specialist Laboratory Services is recognised as a leading UK reference centre for veterinary endocrinology and specialist biochemical and hormone assays 


 
   
  High Sensitivity Oestradiol Assay
 

Increased accuracy for the detection of ovarian function in females (GnRH stimulation) in cats and dogs and the diagnosis of Sertoli cell tumours in dogs (Oestradiol)

GnRH Stimulation Test Protocol

1) Take a basal blood sample.
2) Inject IV 0.32 µg Buserelin (Receptal, Hoechst). (A very small volume of drug is required (83µL), therefore dilute the drug 1:10 with sterile water for injection and then inject 0.83mL).
3) Take a further blood sample 3 hours later.
4) Send both samples for analysis of low level Oestradiol.

Sample requirement:  1 ml serum or plasma.

Low level Oestradiol is run on Monday with results ready Tuesday.

   
  SHAP Sex Hormone Alopecia Profile
 
  • Adrenal Sex Hormone Imbalance
  • Congenital Adrenal Hyperplasia
  • Atypical Cushings Disease
  • Unwanted Sexual Attraction in Male Dogs

Testing Protocol
The adrenal sex hormone status can be investigated by performing an ACTH stimulation test. 17-OH-Progesterone (OHP) has been identified as the most sensitive marker and this along with Cortisol is measured before and after a standard ACTH stimulation test.

SHAP Reference Range
In normal dogs the basal OHP level is less than 3.0 nmol/L (usually undetectable (<1.0 nmol/L)) and following stimulation with ACTH normal dogs show an increase in OHP up to 4.0 nmol/L. Dogs with a possible sex hormone imbalance often have a raised basal OHP level and show a significant increase after stimulation. Cortisol levels must be normal before and after ACTH.

Sample requirements are 2 x 0.5 mL serum or plasma

   
 
 
   
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"The Hormone Lab"
     
  Oestradiol
  SHAP Profile
 
     
     
     
     
     
     
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